Título principal
Influência de combo microbiano na caracterização do farelo de arroz fermentado [recurso eletrônico] / Antônio de Oliveira Vieira ; orientador, José Luiz Pedreira Moutiño ; coorientadora, Scheila Anelise Pereira Dutra
Data de publicação
2024
Descrição física
62 p. : il.
Nota
Disponível somente em versão on-line.
Dissertação (mestrado) – Universidade Federal de Santa Catarina, Centro de Ciências Agrárias, Programa de Pós-Graduação em Aquicultura, Florianópolis, 2024.
Inclui referências.
Influência de combo microbiano na caracterização do farelo de arroz fermentado [recurso eletrônico] / Antônio de Oliveira Vieira ; orientador, José Luiz Pedreira Moutiño ; coorientadora, Scheila Anelise Pereira Dutra
Data de publicação
2024
Descrição física
62 p. : il.
Nota
Disponível somente em versão on-line.
Dissertação (mestrado) – Universidade Federal de Santa Catarina, Centro de Ciências Agrárias, Programa de Pós-Graduação em Aquicultura, Florianópolis, 2024.
Inclui referências.
Abstract: In aquaculture, the search for innovative strategies to improve the inclusion of ingredients in diets is being investigated. Rice bran (RBM) is a viable alternative and can be enriched through fermentation. However, proper development of fermentation methods and selection of probiotic microbial strains is essential. The aim of this study was to evaluate the fermentation of FA (Oryza sativa) by a microbial combo (CM). In trial I, the selection of lactic acid bacteria (LAB) took place in the fermentation of FA individually, for 48 hours at 36°C. The criteria evaluated were pH, colony-forming unit (CFU) count and antagonism test. The CM was composed of: Bacillus subtilis, Saccharomyces cerevisae, Lactobacillus plantarum, Lactobacillus acidophilus and Lactobacillus rhamnosus. In trial II, FA was fermented in two groups: CM and a control with natural fermentation (CFN), for 48 hours at 36°C. The pH, CFU counts of (BAL), total heterotrophic bacteria (BHT) and total yeasts (LT) were assessed. The FA fermented by CM (FACM) was also characterized in relation to the FA in natura (FAIN). In the FACM group (4.12±0.04) there was a 24% drop in pH compared to CFN (5.42±0.28). There was an increase in BAL CFU (p<0.05) with the highest count obtained at 24h and 48h with CM (9.52±0.07) compared to CFN (7.24±0.15), an increase of 23.95%. For BHT, the FACM after 48 hours showed an increase of 17% (8.46±0.31) compared to the CFN (7.07±0.11). For LT, the results were similar to BAL, with higher CFU g-1 after 48h in FACM or CFN. FACM had a higher concentration of yeasts compared to CFN. The characterization of FACM showed significant changes in its physicochemical composition, macronutrients and micronutrients. There was a 40% and 6% increase in crude fiber (CF) and crude protein (CP), respectively. In addition, there was a 2.66% reduction in the content of ether extract (EE), 1.91% in mineral matter (MM), 3.1% in phosphate, 3.1% in phosphorus and 4.48% in potassium. Sodium, iron and calcium content increased by 43.03%, 74.58% and 60.77% respectively in FACM compared to FAIN. In terms of essential amino acids, there was a significant increase of 15.4% in Tryptophan, 10.25% in Valine, 9.5% in Leucine, 8.6% in Lysine, 6.9% in Phenylalanine and 5.9% in Isoleucine. And a significant reduction of 5.77% in Arginine. For the non-essential amino acids, there was a significant increase of: 10.6% for Glycine, 9.8% for Alanine, 9.1% for Proline, 7.36% for Aspartic Acid, 6.25% for Serine and 6.19% for Glutamic Acid. In addition, in this same FACM product, the crude protein content increased significantly by 8.83% and the total sum of amino acids by 6.88% compared to FAIN. Scanning electron microscopy (SEM) showed changes in porosity, microstructure, increased particle fragmentation, visualization of the crystalline calcium structure and the presence of probiotics from the Lactobacillaceae, Saccharomycetaceae and Bacillaceae families. Therefore, fermentation by CM promoted desirable quality to FA for formulating diets and makes it possible to reuse this coproduct, which is rich in nutrients and biological value.